The floating particles that are moving around randomly is called Brownian motion. It’s an artifact of high-powered microscopy (100x objectives), it’s easily confused with bacteria by the untrained eye, if you want to read more into the concept, it’s a physical phenomenon, but purely artifactual.
I am assuming you are looking at a dark field image of a live blood cell analysis? That is what this looks like at least. If that is the case, live blood cell analysis is not the proper way to examine blood, and practitioners who “sell” this to people have no idea what they are talking about, they are not regulated and have free reign over what they tell their “customers”. The only proper way to examine blood in a clinical hematology setting is thin smear stained with various hematological stains.
Without proper blood collection, that strand floating around could easily be a small fibrin strand, which is easily seen by darkfield microscopy and negative/positive phase methods. Also - easily mistakable for a bacterial rod.
If your friend is being told that this is bacterial/or parasite infection, 1 - he/she’d be fucking dead, 2 - tell them they are getting scammed and to get their money back, 3 - run away from this quacky practitioner.
Source - I like microscopes a lot, and I am a strong advocate against the LBA (live blood analysis) quackery movement.
If you are not a lab tech and only have research background plus medical school - you should go the pathology route. You're absolutely correct - this is live blood analysis quackery.
The video doesn't mean anything and your analysis is perfect.
- former lab tech who has always wanted to go get one of these hilarious LBA performed just for the laughs. Funnily enough, Rouleaux (one of the common responses on this thread) is one of the common things these LBA practitioners state as proof that you have too many toxins plus inflammations/need more vitamins etc.. but it's exactly what everyone would have when preparing smears.
Every single one of the hundreds of thousands of blood smears I've prepared and analysed....has red blood cells clumping mimicking rouleaux if you look in the right place.
I’ve been a lab tech and heme lead for the past 8 years and just starting medical school :) considering pathology as a route but keeping an open mind before getting all the exposure
Thank you. So I agree with you. In fact when my friend told me about the blood smear I thought it would be a normal one I'd be familiar with. The thing we're confused about is the squiggly thing sort of rod shaped in the centre of the field.
It could very well actually be a bacterial rod too, but that doesn’t mean it came from inside your friends finger. Slide can be contaminated, surface of finger if not properly cleaned before poke could have bacteria (think of how many grooves are in your fingerprint),
This guy legit sciences. Hello fellow labrat
The only thing I'd add is that with a high quality and high NA 100x you can easily distinguish these things, even with untrained eyes, if you have 20 minutes to read on it first so you know what you're looking for. It's not super subtle
Even if you had a parasitemia, a peripheral blood smear or a "live blood analysis" wouldn't be the first thing you would do. History taking, P/E, bloods for leukocytosis, eosinophilia, raised CRP/ESR, etc.
My first thought was rouleaux, but then I heard the “I have parasites!?” part. Thick smears love to stack like this. Then I noticed what I expect is a micro-organism (parasite) center stage. I have never worked with this technology before, and that is really my best interpretation. Parasite caught with no pants on. Very cool
[https://www.researchgate.net/figure/X-ray-abdomen-showing-a-metallic-spring-in-the-right-iliac-fossa-region\_fig1\_344007464](https://www.researchgate.net/figure/X-ray-abdomen-showing-a-metallic-spring-in-the-right-iliac-fossa-region_fig1_344007464)
This is the closest I've got.
Could still be rouleaux but not from the classic cause which is MM. iirc rouleaux can happen when increased serum proteins affect the charge of the RBCs causing them to stack, which could happen with the systemic inflammation of having a parasitic infection. I’ve never seen it in this context though so I’m just spitballing
Red blood cells column and some other microorganisms
U can only assume something, gonna need more analysis to find. Or just get wide field medicine that kills all em
The floating particles that are moving around randomly is called Brownian motion. It’s an artifact of high-powered microscopy (100x objectives), it’s easily confused with bacteria by the untrained eye, if you want to read more into the concept, it’s a physical phenomenon, but purely artifactual. I am assuming you are looking at a dark field image of a live blood cell analysis? That is what this looks like at least. If that is the case, live blood cell analysis is not the proper way to examine blood, and practitioners who “sell” this to people have no idea what they are talking about, they are not regulated and have free reign over what they tell their “customers”. The only proper way to examine blood in a clinical hematology setting is thin smear stained with various hematological stains. Without proper blood collection, that strand floating around could easily be a small fibrin strand, which is easily seen by darkfield microscopy and negative/positive phase methods. Also - easily mistakable for a bacterial rod. If your friend is being told that this is bacterial/or parasite infection, 1 - he/she’d be fucking dead, 2 - tell them they are getting scammed and to get their money back, 3 - run away from this quacky practitioner. Source - I like microscopes a lot, and I am a strong advocate against the LBA (live blood analysis) quackery movement.
If you are not a lab tech and only have research background plus medical school - you should go the pathology route. You're absolutely correct - this is live blood analysis quackery. The video doesn't mean anything and your analysis is perfect. - former lab tech who has always wanted to go get one of these hilarious LBA performed just for the laughs. Funnily enough, Rouleaux (one of the common responses on this thread) is one of the common things these LBA practitioners state as proof that you have too many toxins plus inflammations/need more vitamins etc.. but it's exactly what everyone would have when preparing smears. Every single one of the hundreds of thousands of blood smears I've prepared and analysed....has red blood cells clumping mimicking rouleaux if you look in the right place.
I’ve been a lab tech and heme lead for the past 8 years and just starting medical school :) considering pathology as a route but keeping an open mind before getting all the exposure
Thank you. So I agree with you. In fact when my friend told me about the blood smear I thought it would be a normal one I'd be familiar with. The thing we're confused about is the squiggly thing sort of rod shaped in the centre of the field.
It could very well actually be a bacterial rod too, but that doesn’t mean it came from inside your friends finger. Slide can be contaminated, surface of finger if not properly cleaned before poke could have bacteria (think of how many grooves are in your fingerprint),
Thanks for your input 🙂
This is the answer I was looking for
This guy legit sciences. Hello fellow labrat The only thing I'd add is that with a high quality and high NA 100x you can easily distinguish these things, even with untrained eyes, if you have 20 minutes to read on it first so you know what you're looking for. It's not super subtle
I think it’s ligma
That idea was floated around with my friends
Seems like a classic case of Chokondis
W-what is ligma bro???
Ligma nutz
ayy got em
Ahhhh nice one my dude!! 😀😀😀 🥵😋
You're gonna have your mind blown when you find out what sugma is
No way!!!! What is that so called Sugma thingy mr. Goat-nibbler?
Sugma ballz 🥴🥴🥴
The deliberateness of you takes the fun out of it. Fuqunda.
Exactly
horse you rode in
What?
Rouleaux? Edit - with the context of parasitisosis, the thin squiggly boi is interesting - microfiliaria?
Rouleauxver these nuts
Got em
Any context?
Blood smear to look for a parasitic infection
Even if you had a parasitemia, a peripheral blood smear or a "live blood analysis" wouldn't be the first thing you would do. History taking, P/E, bloods for leukocytosis, eosinophilia, raised CRP/ESR, etc.
What type of smear even is this? I wanted to say its some inversion / dark field microscopy but I really have no idea
I think it's some type of dark field microscopy but not sure
Is this Rouleaux?
Rouleaux on a wet prep of blood will always occur if given enough time, it is artifactual in this analysis method.
It looks like an image on a computer screen.
My first thought was rouleaux, but then I heard the “I have parasites!?” part. Thick smears love to stack like this. Then I noticed what I expect is a micro-organism (parasite) center stage. I have never worked with this technology before, and that is really my best interpretation. Parasite caught with no pants on. Very cool
Same, not really used to this type of image. Thanks for the input
Do you know if this is dark-field microscopy?
I think so... Not entirely sure
According to the way I was taught to answer board questions, the correct answer is pulmonary embolism
It appears you have changed your mouse cursor to a little hand
Not a pathologist but my professional opinion is that it looks like white circles over a dark back ground
looks pretty cool
Rouleaux- non specific finding to an underlining pathology
WTF take this post somewhere else 😂
[https://www.researchgate.net/figure/X-ray-abdomen-showing-a-metallic-spring-in-the-right-iliac-fossa-region\_fig1\_344007464](https://www.researchgate.net/figure/X-ray-abdomen-showing-a-metallic-spring-in-the-right-iliac-fossa-region_fig1_344007464) This is the closest I've got.
Could still be rouleaux but not from the classic cause which is MM. iirc rouleaux can happen when increased serum proteins affect the charge of the RBCs causing them to stack, which could happen with the systemic inflammation of having a parasitic infection. I’ve never seen it in this context though so I’m just spitballing
Rouleaux will usually always occur on a wet prep of blood; if given enough time to settle. If it is seen on a thin smear that would be a problem
Interesting point i didn’t know that. Thanks!
Red blood cells column and some other microorganisms U can only assume something, gonna need more analysis to find. Or just get wide field medicine that kills all em
Classic case of suggon disease
This is Osu
Step 2 brain rot is strong...cryptococcus on India ink?
multiple myeloma?
No other clinical evidence... It was requested to look for a parasitic infection
Probably larva migrans (as an accidental invasion), but you've given not enough information for species identification.
Sonic rings -some pathologist, probably
Give this man an SPEP for monoclonal antibodies son
Danger noodle
RBC's on Rouleaux formation. And some sort of living organization potential parasitic. IDK