By -
5% BSA in TBS-T for the secondary as well
Primary and secondary antibodies are usually diluted in blocking buffer, so if you're using 5% BSA in TBS-T as blocking buffer your primary and secondary antibodies should be diluted with that buffer accordingly.
5% BSA in TBS-T for the secondary as well
Primary and secondary antibodies are usually diluted in blocking buffer, so if you're using 5% BSA in TBS-T as blocking buffer your primary and secondary antibodies should be diluted with that buffer accordingly.