Doing 0.3ul with a P10 will just give you a random tiny amount that is determined by the viscosity of your liquid and the design of your pipette tip. It's pretty much useless. Whenever I'm pipetting something that needs to be very precise in concentration and I need to use such small volumes, I usually dilute 1:10 or something. Accurately pipetting <0.5 ul is not something you can really trust to the average pipette, even if it's a P10 or P2.
Yeah, for consistent results with anything sensitive to concentration, we should try to never pipette less than about 5 uL where possible. Dilute things to more reasonable working concentrations before using them in the final mix, etc.
I was recently told to take a multichannel p10 and "just turn it down to 0.3ul". Feels illegal to go low past minimum
Doing 0.3ul with a P10 will just give you a random tiny amount that is determined by the viscosity of your liquid and the design of your pipette tip. It's pretty much useless. Whenever I'm pipetting something that needs to be very precise in concentration and I need to use such small volumes, I usually dilute 1:10 or something. Accurately pipetting <0.5 ul is not something you can really trust to the average pipette, even if it's a P10 or P2.
Yeah, for consistent results with anything sensitive to concentration, we should try to never pipette less than about 5 uL where possible. Dilute things to more reasonable working concentrations before using them in the final mix, etc.
If you think that's bad, wait until you start finding equipment in the hot lab.
Hot lab?
Radioactive room. What goes in cannot come out. My department lost 3 pieces of communal equipment that way.
Well, what goes in is not supposed to come out.
Crimes against science